Materials

  • Covaris/Bioruptor/etc. for shearing samples

  • SPRI/AMPure/SeraPure beads for sample purification

  • PEG solution mixture (lacking beads; e.g. 20% PEG, 2.5M NaCl from [Fisher2011])

  • Rare-earth magnet stand (e.g. Ambion AM10055 or NEB S1506S)

  • Kapa Biosystems Library Prep kit (KK8230 & KK8232)

  • Amplification primers for Illumina adapters (includes phosphorothioate bonds)
    • Upper 5’ – AAT GAT ACG GCG ACC ACC GAG A*T- 3’
    • Lower 5’ – CAA GCA GAA GAC GGC ATA CGA GA*T- 3’
  • Illumina TruSeq adapters, BFIDT adapters, iTrue adapters (see Adapter Preparation)

  • Thermal cycler or ThermoMixer with digital temperature control that will accept 1.5 mL tubes.

  • SpeedVac (if you need to dry down libraries)