Materials¶
Covaris/Bioruptor/etc. for shearing samples
SPRI/AMPure/SeraPure beads for sample purification
PEG solution mixture (lacking beads; e.g. 20% PEG, 2.5M NaCl from [Fisher2011])
Rare-earth magnet stand (e.g. Ambion AM10055 or NEB S1506S)
Kapa Biosystems Library Prep kit (KK8230 & KK8232)
- Amplification primers for Illumina adapters (includes phosphorothioate bonds)
- Upper 5’ – AAT GAT ACG GCG ACC ACC GAG A*T- 3’
- Lower 5’ – CAA GCA GAA GAC GGC ATA CGA GA*T- 3’
Illumina TruSeq adapters, BFIDT adapters, iTrue adapters (see Adapter Preparation)
Thermal cycler or ThermoMixer with digital temperature control that will accept 1.5 mL tubes.
SpeedVac (if you need to dry down libraries)