Changelog

v2.1

  • move to read-the-docs.org

v2.0

  • Update for new Kapa kits.

v1.10

  • Remove Fragmentase info. Change end-repair temp. to 20 C.

v1.9

  • Update instructions for adapters to indicate when you would do this (September 24, 2012).

v1.8

  • Add in NEB incubation temps, which are slightly different.

v1.7

  • Fix incorrect total volumes for mixes.

v1.6

  • Minor wording changes for clarity. Change template volume to 15 uL for Phusion PCR alternative.

v1.5

  • Add adapter annealing section.

v1.4

  • Add step telling users to aspirate ddH2O containing DNA after steps 64 and 84.

v1.3

  • Remove all mention of using heat block to dry EtOH from samples.

v1.2

  • Alter PEG solution mix to Fisher’s original; Change ddH2O volumes for each step so we don’t have to add water twice.

v1.1

  • Change volumes of PEG Solution used during cleanup steps to increase post-shear to 3X and others to 2.2X. Final is 0.9X, which may be a little bit low.