Changelog

v2.1

• move to read-the-docs.org

v2.0

• Update for new Kapa kits.

v1.10

• Remove Fragmentase info. Change end-repair temp. to 20 C.

v1.9

• Update instructions for adapters to indicate when you would do this (September 24, 2012).

v1.8

• Add in NEB incubation temps, which are slightly different.

v1.7

• Fix incorrect total volumes for mixes.

v1.6

• Minor wording changes for clarity. Change template volume to 15 uL for Phusion PCR alternative.

v1.5

• Add adapter annealing section.

v1.4

• Add step telling users to aspirate ddH2O containing DNA after steps 64 and 84.

v1.3

• Remove all mention of using heat block to dry EtOH from samples.

v1.2

• Alter PEG solution mix to Fisher’s original; Change ddH2O volumes for each step so we don’t have to add water twice.

v1.1

• Change volumes of PEG Solution used during cleanup steps to increase post-shear to 3X and others to 2.2X. Final is 0.9X, which may be a little bit low.