faircloth-lab illumina library prep protocol¶
Release v2.1. (Changelog)
| Author: | Brant C. Faircloth, Travis C. Glenn, Noor D. White |
|---|---|
| Date: | 23 February 2014 20:17 CST (-0600) |
| Copyright: | This documentation is available under a Creative Commons (CC-BY) license. |
Suggested Reading / References¶
| [Blum2010] | Blumenstiel B et al. 2010. Targeted exon sequencing by in-solution hybrid selection. Curr Protoc Hum Genet Chapter 18:Unit 18.4. |
| [Fair2012] | Faircloth BC, Glenn TC: Not all sequence tags are created equal: designing and validating sequence identification tags robust to indels. PLoS One 7: e42543. |
| [Fish2011] | Fisher S, et al. 2011. A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. Genome Biol 12:R1. |
| [Gnir2009] | Gnirke A et al. 2009. Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing. Nature Biotechnology 2009, 27:182–189. |
| [Kapa2011] | Kapa Biosystems. Kapa Library Preparation Kits, Illumina Series, version 2.11. 2011. Cape Town, South Africa. |
| [NEB2011] | New England Biolabs. NEBNext DNA Sample Prep Master Mix Set 1, Illumina Compatible, version 2.0. 2011. Ipswich, MA, USA. |
Summary¶
Prepare DNA libraries having unique sequence tagged adapters ligated to DNA fragments on a per-library basis.
This protocol incorporates the with-bead AMPure cleanup protocol, initially described in [Fish2011], which conducts all of the library preparation steps in the presence of SPRI/AMPure/SeraPure beads.