faircloth-lab illumina library prep protocol

Release v2.1. (Changelog)

Author:Brant C. Faircloth, Travis C. Glenn, Noor D. White
Date:23 February 2014 20:17 CST (-0600)
Copyright:This documentation is available under a Creative Commons (CC-BY) license.

Suggested Reading / References

[Blum2010]Blumenstiel B et al. 2010. Targeted exon sequencing by in-solution hybrid selection. Curr Protoc Hum Genet Chapter 18:Unit 18.4.
[Fair2012]Faircloth BC, Glenn TC: Not all sequence tags are created equal: designing and validating sequence identification tags robust to indels. PLoS One 7: e42543.
[Fish2011]Fisher S, et al. 2011. A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. Genome Biol 12:R1.
[Gnir2009]Gnirke A et al. 2009. Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing. Nature Biotechnology 2009, 27:182–189.
[Kapa2011]Kapa Biosystems. Kapa Library Preparation Kits, Illumina Series, version 2.11. 2011. Cape Town, South Africa.
[NEB2011]New England Biolabs. NEBNext DNA Sample Prep Master Mix Set 1, Illumina Compatible, version 2.0. 2011. Ipswich, MA, USA.


Prepare DNA libraries having unique sequence tagged adapters ligated to DNA fragments on a per-library basis.

This protocol incorporates the with-bead AMPure cleanup protocol, initially described in [Fish2011], which conducts all of the library preparation steps in the presence of SPRI/AMPure/SeraPure beads.

Protocol info